Alkaline Phosphatase Staining Kit

Cosmo Bio LTD

Catalog No: PMC-AK20

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Alkaline Phosphatase Staining Kit is designed for the rapid and sensitive detection of osteoblasts differentiated in culture from bone marrow cells. Bone forming osteoblasts and bone-degrading osteoclasts can be distinguished by the expression, respectively, of alkaline phosphatase and tartrate-resistant acid phosphatase (TRAP). CosmoBio’s TRAP staining kit (PMC-AK04F-COS) is ideal for rapid and convenient detection of the latter.

Simple staining protocol

1. Remove culture medium. Wash each well 3 times with 1mL PBS.
2. Add 500μL of 10% formalin neutral buffer solution to each well and fix for 20 minutes at room temperature.
3. Remove 10% formalin neutral buffer solution and wash each well 3 times with 2mL deionized water.
4. Dissolve 1 vial of Chromogenic Substrate in 5mL Substrate-containing Buffer.
5. Add 400μL Chromogenic Substrate to each well.
6. Incubate at 37°C for 5-20 minutes. Adjust incubation time until stained.
7. Wash with deionized water to stop the reaction.

References

1. A. S. H. De Jong, M. Van Kessel-Van Vark and A. K. Raap. Sensitivity of various visualization methods for peroxidase and alkaline phosphatase activity in immunoenzyme histochemistry. The Histochemical Journal Volume 17, 1119-1130 (1985)

Citations for Alkaline Phosphatase Staining Kit – 6 Found

Augmenting in vitro osteogenesis of a glycine-arginine-glycine-aspartic-conjugated oxidized alginate-gelatin-biphasic calcium phosphate hydrogel composite and in vivo bone biogenesis through stem cell delivery.
J Biomater Appl. 2016 Nov;31(5):661-673. doi: 10.1177/0885328216667633. Epub 2016 Sep 6. 
- Comments: Cosmo Bio’s Alkaline Phosphatase (ALP) Staining Kit was used to detect osteoblastic activity in a study showing Ebag9-knockout mice exhibit fragile bones with low bone mineral density due to decreased bone formation and enhanced bone resorption arising from a role with interacting protein TMSF1 in osteoblast and osteoclast autophagy.

Lithium-containing surface pre-reacted glass fillers enhance hDPSC functions and induce reparative dentin formation in a rat pulp capping model through activation of Wnt/β-catenin signaling.
Acta Biomater. 2019 Sep 15;96:594-604. doi: 10.1016/j.actbio.2019.06.016. Epub 2019 Jun 15. 
- Comments: Cosmo Bio’s Alkaline Phosphatase (ALP) Staining Kit was used to detect osteoblastic activity in a study showing Ebag9-knockout mice exhibit fragile bones with low bone mineral density due to decreased bone formation and enhanced bone resorption arising from a role with interacting protein TMSF1 in osteoblast and osteoclast autophagy.

EBAG9-deficient mice display decreased bone mineral density with suppressed autophagy.
iScience. 2024 Jan 11;27(2):108871. doi: 10.1016/j.isci.2024.108871. eCollection 2024 Feb 16. 
- Comments: Cosmo Bio’s Alkaline Phosphatase (ALP) Staining Kit was used to detect osteoblastic activity in a study showing Ebag9-knockout mice exhibit fragile bones with low bone mineral density due to decreased bone formation and enhanced bone resorption arising from a role with interacting protein TMSF1 in osteoblast and osteoclast autophagy.

A novel macrolide-Del-1 axis to regenerate bone in old age.
iScience. 2024 Jan 4;27(2):108798. doi: 10.1016/j.isci.2024.108798. eCollection 2024 Feb 16. 
- Comments: Cosmo Bio’s Alkaline Phosphatase (ALP) Staining Kit was used to detect osteoblastic activity in a study revealing a macrolide–DEL-1 axis that can regenerate bone loss due to aging-related disease.

Morphological study of tooth development in podoplanin-deficient mice.
PLoS One. 2017 Feb 21;12(2):e0171912. doi: 10.1371/journal.pone.0171912. eCollection 2017. 
- Comments: Cosmo Bio’s Alkaline Phosphatase Staining Kit was used in an electron microscopic study of podoplanin conditional KO mice, revealing no effect on the development and growth of bones and teeth (including on calcification and alkaline phosphatase activity in cultured calvarial osteoblasts), but a possible effect on formation of the osteocyte network created by cell process elongation.

Unusual effects of a nanoporous gold substrate on cell adhesion and differentiation because of independent multi-branch signaling of focal adhesions.
J Mater Sci Mater Med. 2023 Oct 26;34(11):54. doi: 10.1007/s10856-023-06760-0.